Polito is an Italian surname that may refer to:
People
- Bartolomeo Polito (14th century), Italian painter and artist
- Ciro Polito (born 1979), Italian footballer
- Clemente Polito (17th century), Roman Catholic prelate
- Enzo Polito (1926–2004), Italian water polo player
- Gene Polito (1918–2010), American cinematographer, mechanical engineer and academic
- Joachim Polito (1941), American animator
- Jon Polito (1950–2016), American actor
- Juan Polito (1908-1981), Argentine pianist and orchestra conductor
- Karyn Polito (born 1966), American politician
- Lina Polito (born 1954), Italian actress
- Robert Polito (born 1951), American academic, critic and poet
- Sol Polito (1892–1960), Academy Award nominated cinematographer
- Stephen Polito (1760s–1814), Italian menagerie owner
Other uses
- Polytechnic University of Turin (Politecnico di Torino), a technical university in Turin, Italy