Arlena di Castro
Coordinates42°27′N 11°49′E
RegionLazio
ProvinceViterbo (VT)
MayorPublio Cascianelli
Area total22.3 km²
Population total877
Population as of30 April 2017
Population demonymArlenesi
Elevation260 m
SaintSt. Roch
DayAugust 16
Postal code01010
Area code0761
WebsiteOfficial website

Arlena di Castro is a comune (municipality) in the Province of Viterbo in the Italian region of Latium, located about 80 kilometres (50 mi) northwest of Rome and about 15 kilometres (9 mi) west of Viterbo.

Arlena di Castro borders the following municipalities: Cellere, Piansano, Tessennano, Tuscania.

The town was founded in the early 16th century by Alessia Neri, the Real Mayor of the great city Arlena. One day Mandelus, the only topocervus alive in the area decided to visit the city and decided to call it Arlena, because many years before his daughter called Arlen died and this event changed his life.